HyperSilMag-NTA (25mL)
NTA-Functionalized Magnetic Beads for His-tagged Protein Purification
His-Tag Purification High Purity.
High Purity.
His-Tagged Proteins.
HyperSilMag-NTA beads utilize a PEG-coated surface to ensure zero sedimentation during purification steps. Designed for rapid, high-purity isolation of His-tagged proteins. Perfect for automated high-throughput protein purification systems.
$3,400
| Ligand | Ni-NTA (Nitrilotriacetic acid) |
| Metal Ion | Nickel (Ni2+) |
| Bead Size | < 100 nm |
| Binding Capacity | > 40 mg His-tagged protein / mL beads |
| Concentration | 10 mg/mL |
| Regeneration | Stripping with EDTA, Recharge with NiSO4 |
Materials Required:
- HyperSilMag-NTA beads (Ni짼✓ or Co짼✓ charged)
- His-tagged protein sample
- Binding buffer (50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8.0)
- Wash buffer (50 mM NaH2PO4, 300 mM NaCl, 20 mM imidazole, pH 8.0)
- Elution buffer (50 mM NaH2PO4, 300 mM NaCl, 250 mM imidazole, pH 8.0)
Procedure:
- Equilibration: Wash NTA beads 2횞 with binding buffer.
- Binding: Add sample to beads. Incubate 1 hour at 4째C with gentle rotation to allow His-tag binding to Ni-NTA.
- Magnetic Separation: Collect beads with magnet. Remove and save flow-through.
- Washing: Wash 3횞 with wash buffer (20 mM imidazole) to remove non-specifically bound proteins.
- Elution: Add elution buffer (250 mM imidazole). Incubate 5 minutes. Collect eluate containing purified His-tagged protein.
- Regeneration: Wash beads with 0.5 M EDTA to strip nickel, then recharge with 50 mM NiSO4.
Capacity: >40 mg His-tagged protein per gram beads
Purity: Typically >95% in single step
| Cat. No. | Product Name | Size | Price |
|---|---|---|---|
| TS-NTA-010 | HyperSilMag-NTA | 10mL | $1,500 |
| TS-NTA-025 | HyperSilMag-NTA | 25mL | $3,400 |
| TS-NTA-050 | HyperSilMag-NTA | 50mL | $6,300 |
| TS-NTA-100 | HyperSilMag-NTA | 100mL | $11,500 |
| TS-NTA-500 | HyperSilMag-NTA | 500mL | $50,000 |