HyperSilMag Protein A/G
Functionalized magnetic beads for ligand coupling and protein purification.
Versatile
Custom Ligand
Custom Ligand
Conjugation.
HyperSilMag Carboxyl features a high-density coating of carboxyl groups (-COOH) on its
surface, enabling easy and robust covalent coupling of various biomolecules such as
antibodies and proteins.
Ideal for customized matrices in immunoprecipitation (IP)
and specific protein purification experiments.
| Bead Size | < 100 nm (mean diameter) |
| Ligand | Recombinant Protein A/G |
| Binding Capacity | > 30 mg Human IgG / mL settled beads |
| Magnetic Core | Encapsulated Magnetite (Fe3O4) |
| Concentration | 10 mg/mL |
| Storage | 2-8 C (Do Not Freeze) |
Materials Required:
- HyperSilMag Carboxyl
- Target protein or antibody
- EDC (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide)
- NHS (N-Hydroxysuccinimide)
- MES Buffer (pH 5.0-6.0)
- PBS Buffer (pH 7.4)
- Blocking Buffer (1% BSA in PBS)
Procedure:
- Bead Preparation: Wash beads 3x with MES buffer using magnetic separation.
- Activation: Resuspend beads in MES buffer containing EDC (2 mg/mL) and NHS (5 mg/mL). Incubate for 15 minutes at room temperature.
- Washing: Remove activation solution and wash beads 2x with cold PBS.
- Coupling: Add target protein (0.1-1 mg/mL) in PBS. Incubate 2 hours at room temperature with gentle rotation.
- Blocking: Remove unbound protein and add blocking buffer. Incubate 30 minutes.
- Final Wash: Wash 3x with PBS. Store at 4 C in PBS with 0.02% sodium azide.
Yield: Typically >80% coupling efficiency for most proteins.
| Cat. No. | Product Name | Size | Price |
|---|---|---|---|
| TS-PRO-010 | HyperSilMag Protein A/G | 10 preps | $1,500 |
| TS-PRO-050 | HyperSilMag Protein A/G | 50 preps | $6,300 |
| TS-PRO-100 | HyperSilMag Protein A/G | 100 preps | $11,500 |
| TS-PRO-250 | HyperSilMag Protein A/G | 250 preps | $26,000 |
| TS-PRO-1000 | HyperSilMag Protein A/G | 1000 preps | $90,000 |