HyperSilMag-Carboxylic Acid (50mL)
Carboxyl-Functionalized Magnetic Silica Beads for Rapid Conjugation
Surface Activated Stable & Efficient
Stable & Efficient
Conjugation.
HyperSilMag-Carboxyl beads possess a unique PEG surface layer that eliminates sedimentation. The active carboxyl groups (-COOH) ensure efficient covalent attachment via EDC/NHS. Perfect for creating custom matrices in automated systems.
$6,300
| Surface Group | Carboxylic Acid (-COOH) |
| Bead Size | < 100 nm |
| Core Material | Magnetite (Fe3O4) with Silica Shell |
| Acid Value | > 500 umol/g |
| Concentration | 25 mg/mL |
| Activation Method | EDC / NHS Chemistry |
Materials Required:
- HyperSilMag-Carboxyl beads
- Target protein or amine-containing molecule
- EDC (50 mg/mL in water, prepare fresh)
- NHS (50 mg/mL in water)
- MES buffer pH 5.5
- PBS pH 7.4
Procedure:
- Bead Preparation: Wash carboxyl beads 3횞 with MES buffer pH 5.5.
- Activation: Add EDC (10 mg/mL final) and NHS (10 mg/mL final). Mix 15 minutes at room temperature.
- Washing: Wash activated beads 2횞 with cold MES buffer to remove excess EDC/NHS.
- Coupling: Add target molecule (0.5-2 mg/mL) in PBS pH 7.4. Incubate 2-4 hours at room temperature with rotation.
- Blocking: Add ethanolamine pH 8.5 to 50 mM final concentration. Incubate 30 minutes.
- Final Wash: Wash 3횞 with PBS. Store conjugated beads at 4째C.
Coupling Yield: Typically >80% for most proteins
| Cat. No. | Product Name | Size | Price |
|---|---|---|---|
| TS-COOH-010 | HyperSilMag-Carboxylic Acid | 10mL | $1,500 |
| TS-COOH-025 | HyperSilMag-Carboxylic Acid | 25mL | $3,400 |
| TS-COOH-050 | HyperSilMag-Carboxylic Acid | 50mL | $6,300 |
| TS-COOH-100 | HyperSilMag-Carboxylic Acid | 100mL | $11,500 |
| TS-COOH-500 | HyperSilMag-Carboxylic Acid | 500mL | $50,000 |