HyperSilMag-Amino (25mL)
Amine-Functionalized Magnetic Silica Beads for Ligand Coupling
Surface Activated Versatile Ligand
Versatile Ligand
Coupling.
HyperSilMag-Amino magnetic beads are composed of a magnetic core coated with silica and a PEG layer, functionalized with primary amines.
The PEG layer prevents sedimentation, making these beads ideal for automated workflows.
$3,400
| Surface Group | Amine (-NH2) |
|---|---|
| Bead Size | < 100 nm |
| Core Material | Magnetite (Fe3O4) with Silica Shell |
| Group Density | > 300 µmol/g |
| Concentration | |
| Storage Buffer |
Materials Required:
- HyperSilMag-Amino beads
- Target molecule with carboxyl or carbonyl groups
- Coupling reagent (e.g., glutaraldehyde for proteins)
- Activation buffer (pH 7-8)
- Wash buffer (PBS)
- Blocking solution (Tris or ethanolamine)
Procedure:
- Bead Washing: Wash amino beads 3x with activation buffer.
- Activation: For protein coupling, add 2.5% glutaraldehyde solution. Incubate 1-2 hours at room temperature.
- Washing: Remove glutaraldehyde and wash thoroughly with PBS.
- Coupling: Add target molecule (0.1-1 mg/mL). Incubate overnight at 4°C or 2-4 hours at room temperature with rotation.
- Blocking: Add blocking solution (1 M Tris, pH 8.0). Incubate 30-60 minutes to block unreacted amino groups.
- Final Wash: Wash 3x with PBS. Store at 4°C in PBS with 0.02% sodium azide.
Coupling Efficiency: Typically 70-90% for most biomolecules
| Cat. No. | Product Name | Size | Price |
|---|---|---|---|
| TS-AM-010 | HyperSilMag-Amino | $1,500 | |
| TS-AM-025 | HyperSilMag-Amino | $3,400 | |
| TS-AM-050 | HyperSilMag-Amino | $6,300 | |
| TS-AM-100 | HyperSilMag-Amino | $11,500 | |
| TS-AM-500 | HyperSilMag-Amino | $50,000 |