These beads are coated with Nitrilotriacetic acid (NTA) charged with 니켈 (Ni²⁺), designed for the rapid 정제 of 6xHis-tagged recombinant proteins.
- 샘플 Prep: Lyse cells and clarify lysate. Adjust binding buffer to 10-20mM Imidazole.
- 평형화: Wash beads 2x with 결합 버퍼.
- 결합: 추가 lysate to beads. 인큐베이션 30 min - 1 hour at 4°C.
- Wash: Wash 3x with 세척 버퍼 (containing 20-50mM Imidazole).
- 용출: Elute protein with 용출 버퍼 (250-500mM Imidazole). Isolate 상청액.