These beads are coated with Nitrilotriacetic acid (NTA) charged with Nickel (Ni2+), designed for the rapid purification of 6xHis-tagged recombinant proteins.
- Sample Prep: Lyse cells and clarify lysate. Adjust binding buffer to 10-20mM Imidazole.
- Equilibration: Wash beads 2x with Binding Buffer.
- Binding: Add lysate to beads. Incubate 30 min - 1 hour at 4°C.
- Wash: Wash 3x with Wash Buffer (containing 20-50mM Imidazole).
- Elution: Elute protein with Elution Buffer (250-500mM Imidazole). Isolate supernatant.